hek293 human embryonic kidney epithelial cells  (ATCC)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Dual Functionalized Lactococcus lactis Shows Tumor Antigen Targeting and Cytokine Binding in Vitro

doi: 10.3389/fbioe.2022.822823

Figure Lengend Snippet: The engineered L. lactis strongly and selectively adheres to EpCAM-overexpressing HEK293 cells. Representative confocal microscopy images of adhesion of AFFI co-expressing L. lactis to HEK293 cells transfected with EpCAM (A) or to nontransfected HEK293 cells (B) . Images were quantified by determining the number of adhered bacteria per HEK293 cell in 10 representative micrographs for each experimental condition, using ImageJ (C) . L. lactis strains tested: L. lactis -AFFI-EVA-IRFP ( L. lactis co-expressing AFFI, EVA, and IRFP); L. lactis -AFFI-ZIL-IRFP ( L. lactis co-expressing AFFI, ZIL, and IRFP); L. lactis -AFFI-IRFP ( L. lactis co-expressing AFFI and IRFP; positive control); and L. lactis -IRFP ( L. lactis expressing IRFP only; negative control). DAPI, DAPI channel showing HEK293 cell nuclei; HEK293-EpCAM-sfGFP, green fluorescence channel showing superfolder green fluorescent protein (sfGFP)-labeled EpCAM overexpressed on the surfaces of the HEK293 cells; L. lactis , red fluorescence channel showing L. lactis . Enlarged images show attachment of L. lactis to transfected HEK293 cells with characteristic adhesion pattern (arrows). White squares indicate the enlarged sections of the images. Scale bars, 20 μm.

Article Snippet: Human embryonic kidney epithelial HEK293 cells (CRL-1573; American Type Culture Collection [ATCC], Manassas, Virginia, United States) and human colon adenocarcinoma Caco-2 cells (HTB-37; ATCC) were cultured in Dulbecco’s modified Eagle’s medium with high glucose and GlutaMAX (Gibco, Thermo Fisher Scientific).

Techniques: Confocal Microscopy, Expressing, Transfection, Bacteria, Positive Control, Negative Control, Fluorescence, Labeling

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Dual Functionalized Lactococcus lactis Shows Tumor Antigen Targeting and Cytokine Binding in Vitro

doi: 10.3389/fbioe.2022.822823

Figure Lengend Snippet: The engineered L. lactis specifically adhere to HER2-overexpressing HEK293 cells. Representative confocal microscopy images of adhesion of ZHER co-expressing L. lactis to HEK293 cells transfected with HER2 (A) or to nontransfected HEK293 cells (B) . Images were quantified by determining the number of adhered bacteria per HEK293 cell in 10 representative micrographs for each experimental condition, using ImageJ (C) . L. lactis strains tested: L. lactis -ZHER-EVA-IRFP ( L. lactis co-expressing ZHER, EVA, and IRFP); L. lactis -ZHER-ZIL-IRFP ( L. lactis co-expressing ZHER, ZIL, and IRFP); L. lactis -ZHER-IRFP ( L. lactis co-expressing ZHER and IRFP; positive control); and L. lactis -IRFP ( L. lactis expressing only IRFP; negative control). DAPI, DAPI channel showing HEK293 cell nuclei; HEK293-HER2-mEmerald, green fluorescence channel showing mEmerald-labeled HER2 overexpressed on the surfaces of the HEK293 cells; L. lactis , red fluorescence channel showing L. lactis . Enlarged images show attachment of L. lactis to transfected HEK293 with characteristic adhesion pattern (arrows). White squares indicate enlarged sections of the images. Scale bars, 20 μm.

Article Snippet: Human embryonic kidney epithelial HEK293 cells (CRL-1573; American Type Culture Collection [ATCC], Manassas, Virginia, United States) and human colon adenocarcinoma Caco-2 cells (HTB-37; ATCC) were cultured in Dulbecco’s modified Eagle’s medium with high glucose and GlutaMAX (Gibco, Thermo Fisher Scientific).

Techniques: Confocal Microscopy, Expressing, Transfection, Bacteria, Positive Control, Negative Control, Fluorescence, Labeling